1 1. Heparin Processing
2 2. Serum Processing
3 3. Whole Blood Processing in Citrate Tube
4 4. Tissue Processing
5 5. Na-Heparin Processing
6 6. Serum processing
7 7. Double spin DNA paxgene + Whole blood + Buffy coat, cryovials
8 8. Plasma & Buffy Coat Processing

This page consists of various workflow examples along with the configuration. Workflows can be customized based on lab or study SOPs.

1. Heparin Processing

Receive specimens:

Pool specimens and print labels:

Enter ficoll isolation data:

Pool specimens again:

Derive PBMC:

Pipette PBMC aliquots:

Scan QR Code for all PBMC aliquots:

Below is the zip file for the heparin processing workflow:

2. Serum Processing

Receive specimens:

Rest the blood tube for 60 minutes:

Spin the blood tube for 10 minutes at 200g:

Create serum aliquots:

Scan QR Code for aliquots:

Below is the zip file for the serum processing workflow:

3. Whole Blood Processing in Citrate Tube

Receive specimens:

Centrifuge the citrate tube:

Pipette plasma and buffy coat:

Send plasma tubes for further processing and store buffy coat:

Select the next task:

Store buffy coat:

Enter freeze date and time for the buffy coat:

Centrifuge plasma tubes:

Select next tasks:

Create plasma aliquots, store them and enter the freeze date and time:

Pipette supernatant:

Store supernatant:

Enter freeze date and time:

Below is the file for the whole blood processing in the citrate tube workflow:

4. Tissue Processing

Obtain a fresh specimen:

Assign next tasks:

Select fixation and proceed:

Enter dehydration details:

Enter details for tissue clearing:

Enter details for embedding:

Create DNA and RNA aliquots:

Freeze RNA and DNA aliquots:

Store aliquots:

Below is the file for the whole blood processing in the citrate tube workflow:

5. Na-Heparin Processing

Receive blood tubes:

Select the next task:

Execute dry blood spot:

Select next task:

Enter ficoll isolation details:

Spin ficoll tubes:

Pool Specimens:

Spin ficoll tubes:

Count PBMCs:

The ‘Quantity' field is calculated automatically when you enter values for ‘Cell Counted’ and 'Dilution Factor’.

Create PBMC tubes:

The aliquots count and quantity are calculated automatically based on the parent quantity.
‘Freezing Medium A’ is calculated as aliquots count / 2.

Scan QR code:

Below is the configuration file for the Na-Heparin processing workflow:

6. Serum processing

7. Double spin DNA paxgene + Whole blood + Buffy coat, cryovials

8. Plasma & Buffy Coat Processing

OpenSpecimen

Workflows Library